wiki:DataSet
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Version 1 (modified by Mikael Brandström Durling, 13 years ago) (diff)

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Dataset upload

Some important things to keep in mind while uploading data:

  • Scata will reverese-complement sequences in order to make sure that the 5' primer is found in the 5' end
  • If you give both 5' and 3' primer, any sequence where one of the primers cannot be found will be discarded
  • Scata does not yet understand degenrations in the priming sites. If you have degenerated bases, please lower the matching threshold to account for this, as N, R, Y etc does not match any base.
  • Scata takes priming site sequences, not the primer sequence.
  • Make sure you understand your Quality scores, and adjust the parameters accordingly. The defaults should work ok with new 454 data.