1 | | short read assembler - Velvet is a de novo genomic assembler specially designed for short read sequencing technologies, such as Solexa or 454. Can be used to do whole genome assemblies of Illumina Genome Analyzer (Solexa) data. |
| 1 | short read assembler - Velvet is a de novo genomic assembler specially designed for short read sequencing technologies, such as Solexa. Can be used to do whole genome assemblies of Illumina Genome Analyzer (Solexa) data. |
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| 3 | Velvet can use large amounts of memory. Especially if the dataset is not filtered. A good starting point for a 30M fungal genome could be somewhere in the order of 30GB of ram. |
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| 5 | To obtain the best possible assemblies, you should do proper filtering of the datasets. Low quality reads and adapters must be removed. |