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Changes between Initial Version and Version 1 of DataSet


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Timestamp:
Jul 2, 2011, 10:33:47 PM (13 years ago)
Author:
Mikael Brandström Durling
Comment:

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  • DataSet

    v1 v1  
     1== Dataset upload ==
     2
     3Some important things to keep in mind while uploading data:
     4
     5 * Scata will reverese-complement sequences in order to make sure that the 5' primer is found in the 5' end
     6 * If you give both 5' and 3' primer, any sequence where one of the primers cannot be found will be discarded
     7 * Scata does not yet understand degenrations in the priming sites. If you have degenerated bases, please lower the
     8   matching threshold to account for this, as N, R, Y etc does not match any base.
     9 * Scata takes priming site sequences, not the primer sequence.
     10 * Make sure you understand your Quality scores, and adjust the parameters accordingly. The defaults should work
     11  ok with new 454 data.
     12